Download FONZIE - FONZIE was designed to successively 1) perform a search for markers via the external software Tandem...
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company name:
Jonathan Grandaubert
license: Freeware
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functional limitations:
FONZIE description
FONZIE was designed to successively 1) perform a search for markers via the external software Tandem Repeat Finder, 2) exclude user-defined specific genomic regions, 3) screen for the size and the percent matches of each relevant marker found by Tandem Repeat Finder, 4) evaluate marker specificity (i.e., occurrence of the marker as a single copy in the genome) using BLAST2.0, 5) design minisatellite primer pairs via the external software Primer3, and 6) check the specificity of each final PCR product by BLAST. A final file returns to users all the results required to amplify markers. A biological validation of the approach was performed using the whole genome sequence of the phytopathogenic fungus Leptosphaeria maculans, showing that more than 90% of the minisatellite primer pairs generated by the pipeline amplified a PCR product, 44.8% of which showed agarose-gel resolvable polymorphism between isolates. Segregation analyses confirmed that the polymorphic minisatellites corresponded to single-locus markers.
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FONZIE (Popularity: ) : FONZIE is a simple and robust framework that allows construction of a complex application in remote manner. Initially will act as a simple container and becomes a robust full application after upload of some software modules.
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supported os's
Windows 7
    Windows 7
    Windows Vista Starter
downloads 32
version 12.2010
size in Kb 10
user rating 0/10
our rating 0 Stars
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